Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels.
نویسندگان
چکیده
منابع مشابه
Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels.
Coomassie blue-stained, heat-dried, and computer-imaged two-dimensional gels used to develop comprehensive human protein data bases served as the protein source to generate partial amino acid sequences. The protein spots were collected from multiple gels, rehydrated, concentrated by stacking into a new gel, electroblotted onto inert membranes, and in situ-digested with trypsin. Peptides eluting...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1989
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.86.20.7701